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Location： Home > Phospho Antibodies > MSK1(Phospho-S376) Rabbit mAb

MSK1(Phospho-S376) Rabbit mAb#13389

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Product Detail

Product NameMSK1(Phospho-S376) Rabbit mAb

Clone No.SN63-05

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser376 of human MSK1.

ConjugateUnconjugated

Other Names90 kDa ribosomal protein S6 kinase 5 antibody
EC 2.7.11.1 antibody
KS6A5_HUMAN antibody
MGC1911 antibody
Mitogen and stress activated protein kinase 1 antibody
MSPK1 antibody
Nuclear Mitogen And Stress Activated Protein Kinase 1 antibody
Nuclear mitogen- and stress-activated protein kinase 1 antibody
Ribosomal protein S6 kinase 90kD polypeptide 5 antibody
Ribosomal protein S6 kinase 90kDa antibody
Ribosomal protein S6 kinase 90kDa polypeptide 5 antibody
Ribosomal Protein S6 Kinase Alpha 5 antibody
Ribosomal protein S6 kinase alpha-5 antibody
RLPK antibody
RPS6KA5 antibody
RSK Like Protein Kinase antibody
RSK-like protein kinase antibody
RSKL antibody
S6K alpha 5 antibody
S6K-alpha-5 antibody

Accession NoSwiss-Prot#:O75582

Uniprot O75582

Gene ID 9252;

Calculated MWPredicted band size: 90 kDa

Sdspage MWObserved band size: 90 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes: MSK1(Phospho-S376) Rabbit mAb at 1/1k dilution

Lane 1 : 293 whole cell lysates
Lane 2 : 293 treated with 200nM TPA for 30 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 90 kDa
Observed band size: 90 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded mouse bladder tissue stained for MSK1(Phospho-S376) using 13389 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human liver cancer tissue stained for MSK1(Phospho-phospho S376) using 13389 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence MSK1(Phospho- S376) antibody (13389)

ICC/IF staining of MSK1(Phospho- S376) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13389 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence MSK1(Phospho-S376) antibody (13389)

ICC/IF staining of MSK1(Phospho-S376) in PC-3M cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13389 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The family of ribosomal S6 kinases (Rsks), designated Rsk-1, Rsk-2 and Rsk-3, have been implicated as important signaling intermediates in response to a broad range of ligand-activated receptor tyrosine kinases. A unique feature common to the three members of the Rsk family is that each possesses two non-identical complete kinase catalytic domains. A related S6 kinase, p70 S6 kinase, functions to phosphorylate the S6 protein on ribosomal 40S subunits. p70 S6 kinase b shares high sequence homology with p70 S6 kinase, except in the carboxy terminus where it contains a proline-rich domain that may be involved in SH3 domain containing protein interactions. MSK1 (also designated RLPK) is related to Rsk and p70 S6 kinase family members and is thought to be structurally similar to Rsk family members, but it may be regulated by distinct mechanisms.

If you have published an article using product 13389, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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