Product Detail
Product NameRPA32/RPA2 (Phospho-T21) Rabbit mAb
Clone No.SN06-36
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB
Species ReactivityHu, Ms, Rt
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr21 of human RPA32/RPA2.
ConjugateUnconjugated
Other Names60S acidic ribosomal protein P1 antibody
AA409079 antibody
AI325195 antibody
AU020965 antibody
ik:tdsubc_2g1 antibody
M(2)21C antibody
MGC137236 antibody
OTTHUMP00000004008 antibody
p32 antibody
p34 antibody
RCJMB04_6d17 replication protein A2, 32kDa antibody
REPA2 antibody
Replication factor A protein 2 antibody
Replication protein A 32 kDa subunit antibody
Replication protein A 32kDa subunit antibody
Replication protein A 34 kDa subunit antibody
Replication protein A antibody
Replication Protein A2 (32kDa) antibody
Replication protein A2 antibody
Replication protein A2, 32kDa antibody
RF-A protein 2 antibody
Rf-A2 antibody
RFA antibody
RFA2_HUMAN antibody
RP-A p32 antibody
RP-A p34 antibody
RP21C antibody
RPA 2 antibody
RPA 32 antibody
RPA antibody
Rpa2 antibody
RPA32 antibody
RPA34 antibody
RpLP1 antibody
RpP2 antibody
xx:tdsubc_2g1 antibody
zgc:109822 antibody
Accession NoSwiss-Prot#:P15927
Uniprot
P15927
Gene ID
6118;
Calculated MWPredicted band size: 29 kDa
Sdspage MWObserved band size: 32 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
All lanes: RPA32/RPA2 (Phospho-T21) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with UV for 40 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 32 kDa
Exposure time: 10 seconds
The single-stranded-DNA-binding proteins (SSBs) are essential for DNA function in prokaryotic and eukaryotic cells, mitochondria, phages and viruses. Replication protein A (RPA), a highly conserved eukaryotic protein, is a heterotrimeric SSB. RPA plays an important role in DNA replication, recombination and repair. The binding of human RPA (hRPA) to DNA involves molecular polarity in which initial hRPA binding occurs on the 5' side of a ssDNA substrate and then extends in the 3' direction to create a stably bound hRPA. RPA is a major damage-recognition protein involved in the early stages of nucleotide excision repair. It can also play a role in telomere maintenance. The C-terminus of RPA 32 can specfically intereact with the DNA repair enzyme UNG2 and repair factors XPA and Rad52, each of which functions in a different repair pathway. In addition, RPA 32 binds specifically to the SH2 domain of Stat3 in vivo, and overexpression of RPA 32 corresponds to the augmented growth factor-stimulated tyrosine phosphorylation and transcription activities of Stat3.
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