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Location： Home > Phospho Antibodies > Hormone sensitive lipase (Phospho-S853) Rabbit mAb

Hormone sensitive lipase (Phospho-S853) Rabbit mAb#13391

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Product NameHormone sensitive lipase (Phospho-S853) Rabbit mAb

Clone No.SN06-39

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser853 of human Hormone sensitive lipase.

ConjugateUnconjugated

Other NamesHormone sensitive lipase antibody
Hormone sensitive lipase testicular isoform antibody
Hormone-sensitive lipase antibody
HSL antibody
LHS antibody
Lipase hormone sensitive antibody
LIPE antibody
LIPS_HUMAN antibody

Accession NoSwiss-Prot#:Q05469

Uniprot Q05469

Gene ID 3991;

Calculated MWPredicted band size: 117 kDa

Sdspage MWObserved band size: 84 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : Hormone sensitive lipase (Phospho-S853) Rabbit mAb at 1/1k dilution

Lane 1 : Mouse skeletal muscle tissue lysates
Lane 2 : Mouse skeletal muscle tissue lysates treated with λpp for 1 hour

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 117 kDa
Observed band size: 84 kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human muscle tissue stained for Hormone sensitive lipase (Phospho-Ser853) using 13391 at 1/100 dilution in immunohistochemical analysis.
HSL (hormone-sensitive lipase), a cytosolic neutral lipase regulated by reversible phosphorylation, catalyzes the rate limiting step in triglyceride lipolysis. HSL hydrolyzes stored triglycerides to free fatty acids in adipose and heart tissues. In organs with steroidogenic tissues, such as small intestine, HSL converts cholesteryl esters to free cholesterol for steroid hormone production. HSL is highly expressed in jejunal enterocytes and in the mucosa of the small intestine. Two major isoforms of HSL have been described resulting from the use of alternative translational start codons. The short isoform is expressed in adipose tissue while the long isoform is expressed in steroidogenic tissues such as testis. The long isoform, often referred to as testicular HSL contains an N-terminus of approximately 300 amino acids not present in the short isoform of HSL.

If you have published an article using product 13391, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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