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Location： Home > Phospho Antibodies > STAT1(Phospho-S727) Rabbit mAb

STAT1(Phospho-S727) Rabbit mAb#13392

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Product NameSTAT1(Phospho-S727) Rabbit mAb

Clone No.SN67-04

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser727 of human STAT1.

ConjugateUnconjugated

Other NamesSignal transducer and activator of transcription 1 91kD antibody
CANDF7 antibody
DKFZp686B04100 antibody
IMD31A antibody
IMD31B antibody
IMD31C antibody
ISGF 3 antibody
ISGF-3 antibody
OTTHUMP00000163552 antibody
OTTHUMP00000165046 antibody
OTTHUMP00000165047 antibody
OTTHUMP00000205845 antibody
Signal transducer and activator of transcription 1 91kDa antibody
Signal transducer and activator of transcription 1 antibody
Signal transducer and activator of transcription 1, 91kD antibody
Signal transducer and activator of transcription 1-alpha/beta antibody
STAT 1 antibody
Stat1 antibody
STAT1_HUMAN antibody
STAT91 antibody
Transcription factor ISGF 3 components p91 p84 antibody
Transcription factor ISGF-3 components p91/p84 antibody

Accession NoSwiss-Prot#:P42224

Uniprot P42224

Gene ID 6772;

Calculated MWPredicted band size: 87 kDa

Sdspage MWObserved band size: 87 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : STAT1 (Phospho-Ser727) Rabbit mAb at 1/1k dilution
Lane 1 : 3T3 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with UV for 15min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa

Exposure time: 2 seconds
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for STAT1 (Phospho-S727) using 13392 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for STAT1 (Phospho-S727) using 13392 at 1/100 dilution in immunohistochemical analysis.The picture on the right is blocked with the phospho peptide.
Immunocytochemistry/ Immunofluorescence STAT1 (Phospho-S727) antibody (13392)

ICC/IF staining of STAT1 (Phospho-S727) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13392 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Membrane receptor signaling by various ligands, including interferons and growth hormones such as EGF, induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-a and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 has been shown to be activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.

If you have published an article using product 13392, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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