Product Detail
Product Namec-Myc(Phospho-T58) Rabbit mAb
Clone No.SN60-01
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF, IHC
Species ReactivityHu
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr58 of human c-Myc
ConjugateUnconjugated
Other NamesAvian myelocytomatosis viral oncogene homolog antibody
bHLHe39 antibody
c Myc antibody
Class E basic helix-loop-helix protein 39 antibody
MRTL antibody
Myc antibody
Myc protein antibody
Myc proto oncogene protein antibody
Myc proto-oncogene protein antibody
myc related translation/localization regulatory factor antibody
MYC_HUMAN antibody
Myc2 antibody
MYCC antibody
Niard antibody
Nird antibody
Proto-oncogene c-Myc antibody
Transcription factor p64 antibody
v myc avian myelocytomatosis viral oncogene homolog antibody
v myc myelocytomatosis viral oncogene homolog antibody
Accession NoSwiss-Prot#:P01106
Uniprot
P01106
Gene ID
4609;
Calculated MWPredicted band size: 50 kDa
Sdspage MWObserved band size: 57 kDa
FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes: c-Myc(Phospho-T58) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hours whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 57 kDa
Exposure time: 15 seconds
Formalin-fixed, paraffin-embedded human skin tissue stained for c-Myc(Phospho-T58) using 13394 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence cMyc(Phospho-T58) antibody (13394)
ICC/IF staining of cMyc(Phospho-T58) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13394 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Myc proteins are nuclear proteins with relatively short half lives. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas, while the N-Myc gene has been found amplified in neuroblastomas. The L-Myc gene has been reported to be amplified and expressed at high level in human small cell lung carcinomas. The presence of three sequence motifs in the c-Myc COOH terminus, including the leucine zipper, the helix-loop-helix and a basic region provided initial evidence for a sequence-specific binding function. A basic region helix-loop-helix leucine zipper motif (bHLH-Zip) protein, designated Max, specifically associates with c-Myc, N-Myc and L-Myc proteins. The Myc-Max complex binds to DNA in a sequence-specific manner under conditions where neither Max nor Myc exhibit appreciable binding. Max can also form heterodimers with at least two additional bHLH-Zip proteins, Mad and Mxi1, and Mad-Max dimers have been shown to repress transcription through interaction with mSin3.
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