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Location： Home > Phospho Antibodies > PDGF Receptor beta(Phospho-Y740) Rabbit mAb

PDGF Receptor beta(Phospho-Y740) Rabbit mAb#13396

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Product NamePDGF Receptor beta(Phospho-Y740) Rabbit mAb

Clone No.SN0646

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityMs, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr740 of human PDGF Receptor beta.

ConjugateUnconjugated

Other NamesBeta platelet derived growth factor receptor antibody
Beta-type platelet-derived growth factor receptor antibody
CD 140B antibody
CD140 antigen-like family member B antibody
CD140b antibody
CD140b antigen antibody
IBGC4 antibody
IMF1 antibody
JTK12 antibody
OTTHUMP00000160528 antibody
PDGF R beta antibody
PDGF-R-beta antibody
PDGFR 1 antibody
PDGFR antibody
PDGFR beta antibody
PDGFR1 antibody
PDGFRB antibody
PGFRB_HUMAN antibody
Platelet derived growth factor receptor 1 antibody
Platelet derived growth factor receptor beta antibody
Platelet derived growth factor receptor beta polypeptide antibody

Accession NoSwiss-Prot#:P05622

Uniprot P05622

Gene ID 18596;

Calculated MWPredicted band size: 124 kDa

Sdspage MWObserved band size: 190 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes: PDGF Receptor beta(Phospho-Y740) Rabbit mAb at 1/1k dilution

Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 treated with 40ng/mL PDGF for 30 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 124 kDa
Observed band size: 190 kDa

Exposure time: 15 seconds
Immunocytochemistry/ Immunofluorescence PDGF Receptor beta(Phospho- Y740) antibody (13396)

ICC/IF staining of PDGF Receptor beta(Phospho- Y740) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13396 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Platelet Derived Growth Factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB, and PDGF-BB. These three isoforms bind with different affinities to two receptor types, a and b, which are endowed with protein tyrosine kinase domains and undergo either homo- or heterodimerization as a consequence of ligand binding. Ligand stimulation of PDGFR-b leads to autophosphorylation at Tyr-857, which is the major autophosphorylation site, and Tyr-751, which is the major in vitro phosphorylation site. Autophosphorylation of Tyr-751, which lies in the kinase insert region, is required for binding of phosphatidylinositol-3 kinase to the receptor. These auto-phosphorylation events largely contribute to signal transduction through the PDGF receptor.

If you have published an article using product 13396, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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