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Cdk2(Phospho-Y15) Rabbit mAb#13399

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Product Detail

Product NameCdk2(Phospho-Y15) Rabbit mAb

Clone No.SN72-04

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB,IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr15 of human Cdk2.

ConjugateUnconjugated

Other NamesCdc2 related protein kinase antibody
cdc2-related protein kinase antibody
CDC28 antibody
CDC2A antibody
Cdk 2 antibody
CDK1 antibody
CDK2 antibody
CDK2_HUMAN antibody
CDKN2 antibody
Cell devision kinase 2 antibody
Cell division protein kinase 2 antibody
Cyclin dependent kinase 2 antibody
cyclin dependent kinase 2-alpha antibody
Cyclin-dependent kinase 2 antibody
kinase Cdc2 antibody
MPF antibody
p33 protein kinase antibody
p33(CDK2) antibody

Accession NoSwiss-Prot#:P24941

Uniprot P24941

Gene ID 1017;

Calculated MWPredicted band size: 34 kDa

Sdspage MWObserved band size: 34 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : Cdk2(Phospho-Y15) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Mouse spleen lysates
Lane 3 : NIH/3T3 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 34 kDa
Observed band size: 34 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Cdk2 (Phospho-Y15) using 13399 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse colon tissue stained for Cdk2 (Phospho-Y15) using 13399 at 1/100 dilution in immunohistochemical analysis.
In vertebrates, as in yeast, multiple cyclins have been identified, including a total of eight such regulatory proteins in mammals. In contrast to the situation in yeast, the Cdc2 p34 kinase is not the only catalytic subunit identified in vertebrates that can interact with cyclins. While Cdc2 p34 is essential for the G2 to M transition in vertebrate cells, a second Cdc2-related kinase has also been implicated in cell cycle control. This protein, designated cyclin-dependent kinase 2 (Cdk2) p33, also binds to cyclins and its kinase activity is temporally regulated during the cell cycle. Several additional Cdc2 p34-related cyclin dependent kinases have been identified. These include Cdk3-Cdk8, PCTAIRE-1-3 and KKIALRE.

If you have published an article using product 13399, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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