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Location： Home > Phospho Antibodies > YAP1 (Phospho-S127) Rabbit mAb

YAP1 (Phospho-S127) Rabbit mAb#13401

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Product NameYAP1 (Phospho-S127) Rabbit mAb

Clone No.SN0718

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescHuman breast carcinoma tissue, human kidney tissue, mouse kidney tissue.

ConjugateUnconjugated

Other Names65 kDa Yes associated protein antibody
65 kDa Yes-associated protein antibody
COB1 antibody
YAp 1 antibody
YAP 65 antibody
YAP antibody
YAP1 antibody
YAP1_HUMAN antibody
YAP2 antibody
YAP65 antibody
yes -associated protein delta antibody
Yes associated protein 1 65kDa antibody
Yes associated protein 1 antibody
Yes associated protein 2 antibody
yes associated protein beta antibody
YKI antibody
Yorkie homolog antibody

Accession NoSwiss-Prot#:P46937

Uniprot P46937

Gene ID 10413;

Calculated MWPredicted band size: 54 kDa

Sdspage MWObserved band size: 65kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : YAP1 (Phospho-S127) Rabbit mAb at 1/500 dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 3 : C6 whole cell lysates
Lane 4 : C6 treated with 100nM Calyculin A for 30 minutes whole cell lysates
Lane 5 : NIH/3T3 whole cell lysates
Lane 6 : NIH/3T3 treated with 100nM Calyculin A for 30 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 54 kDa
Observed band size: 65kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human kidney tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse kidney tissue stained for YAP1 (Phospho-S127) using 13401 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence YAP1 (Phospho-S127) antibody (13401)

ICC/IF staining of YAP1 (Phospho-S127) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13401 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The Yes-associated protein, otherwise known as YAP, is a 14-3-3-binding molecule that was originally recognized by virtue of its ability to bind to the SH3 domain of Yes. The binding of YAP to 14-3-3 requires the phosphorylation of a homologous serine residue (Ser 112) in the YAP 14-3-3-binding motif. The highly conserved and ubiquitously expressed 14-3-3 proteins regulate differentiation, cell cycle progression and apoptosis by binding intracellular phosphoproteins involved in signal transduction. YAP may link events at the plasma membrane and cytoskeleton to inhibition of transcription in the nucleus in a manner regulated by 14-3-3 proteins. YAP shares homology with the WW domain of TAZ, transcriptional co-activator with PDZ-binding motif, which functions as a transcriptional co-activator by binding to the PPXY motif present in transcription factors. YAP is expressed at high levels in the lung, placenta, prostate, ovary and testis.

If you have published an article using product 13401, please notify us so that we can cite your literature.

et al,CSRP2 suppresses colorectal cancer progression viap130Cas/Racl axis-meditated ERK,PAK, and HIPPOsignaling pathways. In Theranostics on 2020 Sep 2 by Lixia Chen , Xiaoli Long,et al.. , (2021),
PMID: 33042270
et al,CSRP2 suppresses colorectal cancer progression viap130Cas/Racl axis-meditated ERK,PAK, and HIPPOsignaling pathways. In Theranostics on 2020 Sep 2 by Lixia Chen , Xiaoli Long,et al..PMID:33042270 , (2021),
PMID: 33042270

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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