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Phospho Antibodies > Chk1 (Phospho-S296) Rabbit mAb
Chk1 (Phospho-S296) Rabbit mAb#13402
Yes
All lanes : Chk1 (Phospho-S296) Rabbit mAb at 1/1k dilution
Lane 1 : HEK-293 whole cell lysates
Lane 2 : HEK-293 treated with 200nM Calyculin A for 1 hours whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Chk1 (Phospho-S296) using 13402 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Chk1 (Phospho-S296) using 13402 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Chk1 (Phospho-S296) antibody (13402)
ICC/IF staining of Chk1 (Phospho-S296) in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13402 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
