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Location： Home > Phospho Antibodies > EGFR(Phospho-Y1068) Rabbit mAb

EGFR(Phospho-Y1068) Rabbit mAb#13404

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Product Detail

Product NameEGFR(Phospho-Y1068) Rabbit mAb

Clone No.SD2055

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, FC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGFR.

ConjugateUnconjugated

Other NamesAvian erythroblastic leukemia viral (v erb b) oncogene homolog antibody
Cell growth inhibiting protein 40 antibody
Cell proliferation inducing protein 61 antibody
EGF R antibody
EGFR antibody
EGFR_HUMAN antibody
Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody
Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody
Epidermal growth factor receptor antibody
erb-b2 receptor tyrosine kinase 1 antibody
ERBB antibody
ERBB1 antibody
Errp antibody
HER1 antibody
mENA antibody
NISBD2 antibody
Oncogen ERBB antibody
PIG61 antibody
Proto-oncogene c-ErbB-1 antibody
Receptor tyrosine protein kinase ErbB 1 antibody
Receptor tyrosine-protein kinase ErbB-1 antibody
SA7 antibody
Species antigen 7 antibody
Urogastrone antibody
v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody
wa2 antibody
Wa5 antibody

Accession NoSwiss-Prot#:P00533

Uniprot P00533

Gene ID 1956;

Calculated MWPredicted band size: 134 kDa

Sdspage MWObserved band size: 170 kDa

FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : EGFR(Phospho-Y1068) Rabbit mAb at 1/1k dilution

Lane 1 : A431 whole cell lysates
Lane 2 : A431 treated with 100ng/mL EGF for 30 minutes whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 134 kDa
Observed band size: 170 kDa

Exposure time: 10 seconds
Immunocytochemistry/ Immunofluorescence EGFR(Phospho-Y1068) antibody (13404)

ICC/IF staining of EGFR(Phospho-Y1068) in A431 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13404 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence EGFR(Phospho-Y1068) antibody (13404)

ICC/IF staining of EGFR(Phospho-Y1068) in MCF-7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13404 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Flow Cytometry (Intracellular) EGFR(Phospho-Y1068) antibody (13404)
Flow cytometric analysis of Hela cells with EGFR(Phospho-Y1068) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody，black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Epidermal growth factor mediates its effects on cell growth through its inter-action with a cell surface glycoprotein designated the EGF receptor. Binding of EGF or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase activity intrinsic to the EGF receptor. The carboxy terminal tyrosine residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of autophosphorylation, which occurs as a result of EGF binding. Once activated, EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2 through direct interactions with Tyr 1068 and Tyr 1086 and through indirect interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068 and Tyr 1086 is required for conformational change in the C-terminal tail of the EGF receptor.

If you have published an article using product 13404, please notify us so that we can cite your literature.

et al,Lung cancer cells that survive ionizing radiation show increased integrin 伪2尾1-and EGFR-dependent invasiveness. In PLoS One on 2013 Aug 8 by Xue Li, Seiichiro Ishihara, et al..PMID:23951036 , (2013),
PMID: 23951036
et al,CD146 is a coreceptor for VEGFR-2 in tumor angiogenesis. In Blood on 2012 Sep 13 by Tianxia Jiang, Jie Zhuang, et al..PMID: 22718841 , (2012),
PMID: 22718841

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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