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Location： Home > Phospho Antibodies > Cyclin E1(Phospho-T77) Rabbit mAb

Cyclin E1(Phospho-T77) Rabbit mAb#13405

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Product NameCyclin E1(Phospho-T77) Rabbit mAb

Clone No.SD2025

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr77 of human Cyclin E1.

ConjugateUnconjugated

Other NamesCCNE antibody
Ccne1 antibody
CCNE1_HUMAN antibody
cyclin E variant ex5del antibody
cyclin E variant ex7del antibody
Cyclin E1 antibody
Cyclin Es antibody
Cyclin Et antibody
CyclinE antibody
G1/S specific cyclin E antibody
G1/S-specific cyclin-E1 antibody

Accession NoSwiss-Prot#:P24864

Uniprot P24864

Gene ID 898;

Calculated MWPredicted band size: 47 kDa

Sdspage MWObserved band size: 48 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Cyclin E1 (Phospho-T77) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : HepG2 whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
Exposure time: 15 seconds
All lanes : Cyclin E1 (Phospho-T77) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : K562 treated with 100nM Calyculin A for 30min whole cell lysates
Lane 3 : 293T whole cell lysates
Lane 4 : 293T treated with 100nM Calyculin A for 30min whole cell lysates
Lane 5 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 48 kDa
Exposure time: 8 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Cyclin E1 (Phospho-T77) using 13405 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human colon cancer tissue stained for Cyclin E1 (Phospho-T77) using 13405 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Cyclin E1 (Phospho-T77) antibody (13405)

ICC/IF staining of Cyclin E1(Phospho-T77) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13405 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
The cells were incubated 30 minutes at room temperature with phalloidin tritc .
Nuclei were counterstained with DAPI.
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. The cyclin E/E2F complex is detected primarily during the G1 phase of the cell cycle and decreases as cells enter S phase. E2F is known to be a critical transcription factor for expression of several S phase specific proteins.

If you have published an article using product 13405, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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