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Location： Home > Phospho Antibodies > Smad2 (Phospho-S250) Rabbit mAb

Smad2 (Phospho-S250) Rabbit mAb#13406

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Product NameSmad2 (Phospho-S250) Rabbit mAb

Clone No.SD207-1

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser250 of human Smad2.

ConjugateUnconjugated

Other NamesDrosophila, homolog of, MADR2 antibody
hMAD-2 antibody
HsMAD2 antibody
JV18 antibody
JV18-1 antibody
JV181 antibody
MAD antibody
MAD homolog 2 antibody
MAD Related Protein 2 antibody
Mad-related protein 2 antibody
MADH2 antibody
MADR2 antibody
MGC22139 antibody
MGC34440 antibody
Mother against DPP homolog 2 antibody
Mothers against decapentaplegic homolog 2 antibody
Mothers against decapentaplegic, Drosophila, homolog of, 2 antibody
Mothers against DPP homolog 2 antibody
OTTHUMP00000163489 antibody
Sma and Mad related protein 2 antibody
Sma- and Mad-related protein 2 MAD antibody
SMAD 2 antibody
SMAD family member 2 antibody
SMAD, mothers against DPP homolog 2 antibody
SMAD2 antibody
SMAD2_HUMAN antibody

Accession NoSwiss-Prot#:Q15796

Uniprot Q15796

Gene ID 4087;

Calculated MWPredicted band size: 52 kDa

Sdspage MWObserved band size: 62kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Smad2 (Phospho-Ser250) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Brain lysates
Lane 2 : C6 whole cell lysates
Lane 3 : Hela whole cell lysates
Lane 4 : Hela treated with 200nM TPA for 30 min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 62kDa

Exposure time: 4 seconds
Formalin-fixed, paraffin-embedded human hepatocellular carcinoma tissue stained for Smad2 (Phospho-S250) using 13406 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Smad2 (Phospho-S250) antibody (13406)

ICC/IF staining of Smad2 (Phospho-S250) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13406 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control.
Nuclei were counterstained with DAPI.
Smad proteins, the mammalian homologs of the Drosophila mothers against decapentaplegic (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1) and Smad5 are effectors of BMP-2 and BMP-4 function, while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and Activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to Activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad proteins.

If you have published an article using product 13406, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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