Product Detail
Product NameMEK1 (Phospho-T292) Rabbit mAb
Clone No.SD2088
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB
Species ReactivityHu
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr292 of human MEK1
ConjugateUnconjugated
Other NamesDual specificity mitogen activated protein kinase kinase 1 antibody
Dual specificity mitogen-activated protein kinase kinase 1 antibody
ERK activator kinase 1 antibody
MAP kinase kinase 1 antibody
MAP kinase/Erk kinase 1 antibody
MAP2K1 antibody
MAPK/ERK kinase 1 antibody
MAPKK 1 antibody
MAPKK1 antibody
MEK 1 antibody
Mek1 antibody
MEKK1 antibody
Mitogen activated protein kinase kinase 1 antibody
MKK 1 antibody
MKK1 antibody
MP2K1_HUMAN antibody
PRKMK1 antibody
Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
Protein kinase mitogen activated, kinase 1 antibody
Accession NoSwiss-Prot#:Q02750
Uniprot
Q02750
Gene ID
5604;
Calculated MWPredicted band size: 43 kDa
Sdspage MWObserved band size: 43 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
All lanes: MEK1 (Phospho-T292) Rabbit mAb at 1/1k dilution
Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 100ng/mL Nocodazole for 17 hours whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Exposure time: 10 seconds
A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK 5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
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