Product Detail
Product NameAMPK alpha 1 (Phospho-S496) Rabbit mAb
Clone No.SD0810
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF
Species ReactivityHu
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser496 of human AMPK alpha 1.
ConjugateUnconjugated
Other Names5 AMP activated protein kinase alpha 1catalytic subunit antibody
5 AMP activated protein kinase catalytic alpha 1 chain antibody
5' AMP activated protein kinase catalytic subunit alpha 1 antibody
5'-AMP-activated protein kinase catalytic subunit alpha-1 antibody
AAPK1 antibody
AAPK1_HUMAN antibody
ACACA kinase antibody
acetyl CoA carboxylase kinase antibody
AI194361 antibody
AI450832 antibody
AL024255 antibody
AMP -activate kinase alpha 1 subunit antibody
AMP-activated protein kinase, catalytic, alpha -1 antibody
AMPK 1 antibody
AMPK alpha 1 antibody
AMPK alpha 1 chain antibody
AMPK antibody
AMPK subunit alpha-1 antibody
AMPK1 antibody
AMPKa1 antibody
AMPKalpha1 antibody
C130083N04Rik antibody
cb116 antibody
EC 2.7.11.1 antibody
HMG CoA reductase kinase antibody
HMGCR kinase antibody
hormone sensitive lipase kinase antibody
Hydroxymethylglutaryl CoA reductase kinase antibody
im:7154392 antibody
kinase AMPK alpha1 antibody
MGC33776 antibody
MGC57364 antibody
OTTHUMP00000161795 antibody
OTTHUMP00000161796 antibody
PRKAA 1 antibody
PRKAA1 antibody
Protein kinase AMP activated alpha 1 catalytic subunit antibody
SNF1-like protein AMPK antibody
SNF1A antibody
Tau protein kinase PRKAA1 antibody
wu:fa94c10 antibody
Accession NoSwiss-Prot#:Q13131
Uniprot
Q13131
Gene ID
5562;
Calculated MWPredicted band size: 64 kDa
Sdspage MWObserved band size: 64 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : AMPK alpha 1 (Phospho-Ser496) Rabbit mAb at 1/1k dilutionLane 1 : Hela whole cell lysatesLane 2 : Hela treated with UV for 15min whole cell lysatesLane 3 : HUVEC whole cell lysatesLane 4 : HUVEC treated with UV for 15min whole cell lysatesLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 64 kDa Observed band size: 64 kDaExposure time: 4 seconds
Immunocytochemistry/ Immunofluorescence AMPK alpha 1 (Phospho-S496) antibody (13409)
ICC/IF staining of AMPK alpha 1 (Phospho-S496) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13409 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence AMPK alpha 1 (Phospho-S496) antibody (13409)
ICC/IF staining of AMPK alpha 1 (Phospho-S496) in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13409 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
AMPK (5'-AMP-activated protein kinase) is a heterotrimeric complex comprising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. AMPKα1 (5'-AMP-activated protein kinase catalytic subunit alpha-1), also known as PRKAA1, is a 559 amino acid protein that belongs to the CAMK Ser/Thr protein kinase family and protein kinase superfamily. Highly phosphorylated, AMPKα1 exists as two alternatively spliced isoforms and is encoded by a gene that maps to human chromosome 5p13.1.
If you have published an article using product 13409, please notify us so that we can cite your literature.
Yes
