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Location： Home > Phospho Antibodies > CDC37 (Phospho-S13) Rabbit mAb

CDC37 (Phospho-S13) Rabbit mAb#13411

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Product Detail

Product NameCDC37 (Phospho-S13) Rabbit mAb

Clone No.SD08-50

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser13 of human CDC37.

ConjugateUnconjugated

Other NamesCDC 37 antibody
Cdc37 antibody
CDC37 cell division cycle 37 homolog antibody
CDC37 cell division cycle 37 S cerevisiae homolog antibody
CDC37 cell division cycle 37, S cerevisiae, homolog of antibody
Cdc37 homolog antibody
CDC37 protein antibody
CDC37_HUMAN antibody
CDC37A antibody
cell division cycle 37 antibody
Cell division cycle 37 homolog antibody
Hsp90 chaperone protein kinase targeting subunit antibody
Hsp90 chaperone protein kinase targeting subunit p50Cdc37 antibody
Hsp90 chaperone protein kinase-targeting subunit antibody
Hsp90 co chaperone Cdc37 antibody
Hsp90 co-chaperone Cdc37 antibody
p50 antibody
p50Cdc37 antibody
S cerevisiae hypothetical protein CDC37 antibody

Accession NoSwiss-Prot#:Q16543

Uniprot Q16543

Gene ID 11140;

Calculated MWPredicted band size: 44 kDa

Sdspage MWObserved band size: 44 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : CDC37 (Phospho-S13) Rabbit mAb at 1/1k dilution

Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 whole cell lysates treated with λpp for 1 hour
Lane 3 : Jurkat whole cell lysates
Lane 4 : Jurkat whole cell lysates treated with λpp for 1 hour

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 44 kDa

Exposure time: 10 seconds
All lanes : c Raf (Phospho-S43) Rabbit mAb at 1/1k dilution Lane 1 : 3T3 whole cell lysates Lane 2 : Mouse Heart lysates Lane 3 : Rat Brain lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 65-75 kDa Exposure time: 10 seconds
All lanes : c Raf (Phospho-S43) Rabbit mAb at 1/1k dilution Lane 1 : MCF7 whole cell lysates Lane 2 : MCF7 treated with 200nM PMA for 30min whole cell lysates Lane 3 : MCF7 treated with Lambda Protein Phosphatase for 30min whole cell lysates Lysates/proteins at 20 µg per lane. Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution Predicted band size: 73 kDa Observed band size: 65-75 kDa Exposure time: 7 seconds
Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division cycle (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with Hsp90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

If you have published an article using product 13411, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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