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Location： Home > Phospho Antibodies > Raf1 (Phospho-S259) Rabbit mAb

Raf1 (Phospho-S259) Rabbit mAb#13413

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Product NameRaf1 (Phospho-S259) Rabbit mAb

Clone No.SD85-07

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser259 of human Raf1

ConjugateUnconjugated

Other Namesc Raf antibody
C-raf antibody
C-Raf proto-oncogene, serine/threonine kinase antibody
CMD1NN antibody
Craf 1 transforming gene antibody
cRaf antibody
Craf1 transforming gene antibody
EC 2.7.11.1 antibody
kinase Raf1 antibody
Murine sarcoma 3611 oncogene 1 antibody
NS5 antibody
Oncogene MIL antibody
Oncogene RAF1 antibody
OTTHUMP00000160218 antibody
OTTHUMP00000207813 antibody
OTTHUMP00000209389 antibody
Protein kinase raf 1 antibody
Proto-oncogene c-RAF antibody
Raf 1 antibody
Raf 1 proto oncogene serine/threonine kinase antibody
RAF antibody
Raf proto oncogene serine/threonine protein kinase antibody
RAF proto-oncogene serine/threonine-protein kinase antibody
RAF-1 antibody
RAF1 antibody
RAF1_HUMAN antibody
Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody
TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody
v raf 1 murine leukemia viral oncogene homolog 1 antibody
v-raf murine sarcoma viral oncogene homolog 1 antibody
v-raf-1 murine leukemia viral oncogene-like protein 1 antibody
vraf1 murine leukemia viral oncogene homolog 1 antibody

Accession NoSwiss-Prot#:P04049

Uniprot P04049

Gene ID 5894;

Calculated MWPredicted band size: 73 kDa

Sdspage MWObserved band size: 73 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : Raf1 (Phospho-S259) Rabbit mAb at 1/1k dilution

Lane 1 : NIH/3T3 whole cell lysates
Lane 2 : NIH/3T3 whole cell lysates treated with λpp for 1 hour
Lane 3 : HEK-293 whole cell lysates
Lane 4 : HEK-293 whole cell lysates treated with λpp for 1 hour

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 73 kDa
Observed band size: 73 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded rat skeletal muscle tissue stained for Raf1 (Phospho-S259) using 13413 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Raf1 (Phospho-S259) using 13413 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Raf1 (Phospho-S259) antibody (13413)

ICC/IF staining of Raf1 (Phospho-S259) in HEK293 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13413 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Raf-1 is a ubiquitously expressed cytoplasmic protein with intrinsic serine/threonine kinase activity. Raf-1, or c-Raf, is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein is associated with cellular transformation and mitogenesis. Raf-1 is normally suppressed by its regulatory N-terminal domain. Raf-1 is activated in response to a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression. Specifically, Raf-1 is phosphorylated in the catalytic domain at Ser 338 and, to a lesser extent, Ser 339. This phosphorylation requires the co-activation of PI 3-kinase and the Ras signaling pathway. Raf-1 is also phosphorylated on Tyr 340 and 341, which induces the phosphorylation of MEK. Phosphorylation of Ser 621 is essential for the catalytic activity of Raf-1 and downregulation by c-AMP-dependent protein kinase A (PKA). PKA also phosphorylates Raf-1 on Ser 43 and Ser 259. PKA phosphorylation of Ser 259 inhibits Raf-1 and decreases the phosphorylation necessary for Raf-1 activation at Ser 338.

If you have published an article using product 13413, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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