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Location： Home > Phospho Antibodies > Cdc6 (Phospho-S54) Rabbit mAb

Cdc6 (Phospho-S54) Rabbit mAb#13414

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Product Detail

Product NameCdc6 (Phospho-S54) Rabbit mAb

Clone No.SD08-47

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser54 of human Cdc6.

ConjugateUnconjugated

Other NamesCdc 18L antibody
Cdc 6 antibody
CDC18 (cell division cycle 18, S.pombe, homolog) like antibody
CDC18 (S.pombe) antibody
Cdc18 related protein antibody
CDC18(S.pombe) antibody
Cdc18-related protein antibody
Cdc18L antibody
cdc6 antibody
CDC6 cell division cycle 6 homolog antibody
CDC6 related protein antibody
CDC6-related protein antibody
CDC6_HUMAN antibody
Cdc6p antibody
CELL CYCLE CONTROLLER CDC6 antibody
Cell division control protein 6 antibody
Cell division control protein 6 homolog antibody
Cell division cycle 6 homolog (S. cerevisiae) antibody
Cell division cycle 6 homolog antibody
Cell division cycle 6, S. cerevisiae, homolog of antibody
HsCDC 6 antibody
HsCDC18 antibody
HsCDC6 antibody
p62 antibody
p62(cdc 6) antibody
p62(cdc6) antibody

Accession NoSwiss-Prot#:Q99741

Uniprot Q99741

Gene ID 990;

Calculated MWPredicted band size: 63 kDa

Sdspage MWObserved band size: 63 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes: Cdc6 (Phospho-S54) Rabbit mAb at 1/1k dilution

Lane 1 : Jurkat whole cell lysates
Lane 2 : Jurkat treated with FBS whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 63 kDa
Observed band size: 63 kDa

Exposure time: 10 seconds
Immunocytochemistry/ Immunofluorescence Cdc6 (Phospho-S54) antibody (13414)

ICC/IF staining of Cdc6 (Phospho-S54) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13414 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence Cdc6 (Phospho-S54) antibody (13414)

ICC/IF staining of Cdc6 (Phospho-S54) in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13414 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. Cdc25A, Cdc25B and Cdc25C protein Tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory Tyrosine residues. Cdc6 is the human homolog of Saccharomyces cerevisiae Cdc6, which is involved in the initiation of DNA replication. Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90. Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of Saccharomyces cerevisiae Cdc34, which is essential for the G1 to S phase transition. Cdc16 and Cdc27 are components of the APC (x complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation.

If you have published an article using product 13414, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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