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Location： Home > Phospho Antibodies > B Raf(Phospho-T401) Rabbit mAb

B Raf(Phospho-T401) Rabbit mAb#13416

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Product NameB Raf(Phospho-T401) Rabbit mAb

Clone No.JJ08-72

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr401 of human B Raf.

ConjugateUnconjugated

Other NamesFLJ95109 antibody
94 kDa B raf protein antibody
B raf 1 antibody
B Raf proto oncogene serine threonine protein kinase antibody
B Raf proto oncogene, serine/threonine kinase antibody
B RAF1 antibody
B-Raf proto-oncogene serine/threonine-protein kinase (p94) antibody
BRAF 1 antibody
BRAF antibody
BRAF_HUMAN antibody
BRAF1 antibody
cRmil antibody
MGC126806 antibody
MGC138284 antibody
Murine sarcoma viral (v-raf) oncogene homolog B1 antibody
Murine sarcoma viral v raf oncogene homolog B1 antibody
NS7 antibody
Oncogen BRAF antibody
oncogene BRAF1 antibody
p94 antibody
Proto-oncogene B-Raf antibody
Proto-oncogene c-Rmil antibody
RAFB 1 antibody
RAFB1 antibody
RMIL antibody
Serine/threonine-protein kinase B-raf antibody
v raf murine sarcoma viral oncogene homolog B antibody
v raf murine sarcoma viral oncogene homolog B1 antibody
v-Raf murine sarcoma viral oncogene homolog B1 antibody

Accession NoSwiss-Prot#:P15056

Uniprot P15056

Gene ID 673;

Calculated MWPredicted band size: 84 kDa

Sdspage MWObserved band size: 84 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : B Raf(Phospho-T401) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : 3T3 whole cell lysates
Lane 4 : Hela whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa

Exposure time: 8 seconds
All lanes : B Raf(Phospho-T401) Rabbit mAb at 1/1k dilution
Lane 1 : PC12 whole cell lysates
Lane 2 : PC12 treated with 200nM TPA for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 84 kDa
Observed band size: 84kDa

Exposure time: 7 seconds
Formalin-fixed, paraffin-embedded human testis tissue stained for B Raf (Phospho-T401) using 13416 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence B Raf(Phospho-T401) antibody (13416)

ICC/IF staining of B Raf(Phospho-T401) in HEK293 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13416 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. Raf-A, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of Raf-B is highly restricted, with highest levels in the cerebrum and testis.

If you have published an article using product 13416, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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