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Location： Home > Phospho Antibodies > SIRT1(Phospho-T530) Rabbit mAb

SIRT1(Phospho-T530) Rabbit mAb#13417

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Product NameSIRT1(Phospho-T530) Rabbit mAb

Clone No.JJ206-6

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr530 of human SIRT1.

ConjugateUnconjugated

Other Names75SirT1 antibody
hSIR2 antibody
hSIRT1 antibody
HST2, S. cerevisiae, homolog of antibody
NAD dependent deacetylase sirtuin 1 antibody
NAD dependent protein deacetylase sirtuin 1 antibody
OTTHUMP00000198111 antibody
OTTHUMP00000198112 antibody
Regulatory protein SIR2 homolog 1 antibody
SIR1_HUMAN antibody
SIR2 antibody
SIR2 like 1 antibody
SIR2 like protein 1 antibody
SIR2, S.cerevisiae, homolog-like 1 antibody
SIR2-like protein 1 antibody
SIR2ALPHA antibody
SIR2L1 antibody
Sirt1 antibody
SirtT1 75 kDa fragment antibody
Sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) antibody
Sirtuin 1 antibody
Sirtuin type 1 antibody

Accession NoSwiss-Prot#:Q96EB6

Uniprot Q96EB6

Gene ID 23411;

Calculated MWPredicted band size: 82 kDa

Sdspage MWObserved band size: 120 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : SIRT1(Phospho-T530) Rabbit mAb at 1/1k dilution

Lane 1 : 293T whole cell lysates
Lane 2 : 293T treated with 100nM Calyculin A for 30min whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 82 kDa
Observed band size: 120 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human kidney tissue stained for SIRT1 (Phospho-T530) using 13417 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence SIRT1(Phospho-T530) antibody (13417)

ICC/IF staining of SIRT1(Phospho-T530) in HeLa cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13417 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The silent information regulator (SIR2) family of genes are highly-conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lys 382.

If you have published an article using product 13417, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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