Product Detail
Product NamePLK1(Phospho-T210) Rabbit mAb
Clone No.JJ080-9
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB,IHC
Species ReactivityHu, Ms, Rt
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr210 of human PLK1.
ConjugateUnconjugated
Other NamesCell cycle regulated protein kinase antibody
PLK 1 antibody
PLK antibody
PLK-1 antibody
plk1 antibody
PLK1_HUMAN antibody
Polo like kinase 1 antibody
Polo-like kinase 1 antibody
Serine/threonine protein kinase 13 antibody
Serine/threonine protein kinase PLK1 antibody
Serine/threonine-protein kinase 13 antibody
Serine/threonine-protein kinase PLK1 antibody
STPK 13 antibody
STPK13 antibody
Accession NoSwiss-Prot#:P53350
Uniprot
P53350
Gene ID
5347;
Calculated MWPredicted band size: 68 kDa
Sdspage MWObserved band size: 68 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : PLK1(Phospho-T210) Rabbit mAb at 1/500 dilution
Lane 1 : Hela whole cell lysates
Lane 2 : PC12 whole cell lysates
Lane 3 : Mouse cerebellum lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PLK1 (Phospho-T210) using 13421 at 1/100 dilution in immunohistochemical analysis.
Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and CDC5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle and both protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. The phosphorylation of mouse, rat and human Plk on Thr 210 enhances Plk catalytic activity.
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