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Location： Home > Phospho Antibodies > JunD(Phospho-S255) Rabbit mAb

JunD(Phospho-S255) Rabbit mAb#13422

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Product Detail

Product NameJunD(Phospho-S255) Rabbit mAb

Clone No.JJ08-21

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser255 of human JunD.

ConjugateUnconjugated

Other NamesActivator protein 1 antibody
AP 1 antibody
AP1 antibody
Jun D antibody
jun D proto oncogene antibody
Jund antibody
JunD FL isoform antibody
JUND_HUMAN antibody
Transcription factor jun D antibody
Transcription factor jun-D antibody

Accession NoSwiss-Prot#:P17535

Uniprot P17535

Gene ID 3727;

Calculated MWPredicted band size: 38 kDa

Sdspage MWObserved band size: 42 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : JunD(Phospho-S255) Rabbit mAb at 1/1000 dilution

Lane 1 : Hela whole cell lysates treated with λpp for 1 hour
Lane 2 : Hela whole cell lysates
Lane 3 : PC12 whole cell lysates
Lane 4 : NIH/3T3 whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 38 kDa
Observed band size: 42 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for JunD (Phospho-S255) using 13422 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse liver tissue stained for JunD (Phospho-S255) using 13422 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse testis tissue stained for JunD (Phospho-S255) using 13422 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence JunD (Phospho-S255) antibody (13422)

ICC/IF staining of JunD(Phospho-S255) in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13422 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The activator protein-1 (AP-1) transcription factor consists of either Jun/Jun homodimers or Fos/Jun heterodimeric complexes. Homo- and heterodimers bind to the TGACTCA consensus sequence present in numerous promoters and initially identified as the phorbol ester tumor promoter response element (TRE). Jun B and Jun D have been shown to be almost identical to c-Jun in their C-terminal regions, which are involved in dimerization and DNA binding, whereas their N-terminal domains, which are involved in transcriptional activation, diverge. All three form heterodimers among themselves and with c-Fos and other members of the Fos gene family. Studies suggest that the two forms of Jun D may be due to internal initiation of translation.

If you have published an article using product 13422, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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