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Location： Home > Phospho Antibodies > AMPK alpha 2(Phospho-S345) Rabbit mAb

AMPK alpha 2(Phospho-S345) Rabbit mAb#13424

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Product NameAMPK alpha 2(Phospho-S345) Rabbit mAb

Clone No.JJ08-19

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser345 of human AMPK alpha 2.

ConjugateUnconjugated

Other Names5'-AMP-activated protein kinase catalytic subunit alpha-2 antibody
AAPK2_HUMAN antibody
ACACA kinase antibody
Acetyl-CoA carboxylase kinase antibody
AMPK alpha 2 chain antibody
AMPK subunit alpha-2 antibody
AMPK2 antibody
AMPKa2 antibody
AMPKalpha2 antibody
HMGCR kinase antibody
Hydroxymethylglutaryl-CoA reductase kinase antibody
PRKAA antibody
PRKAA2 antibody
Protein kinase AMP activated alpha 2 catalytic subunit antibody
Protein kinase AMP activated catalytic subunit alpha 2 antibody

Accession NoSwiss-Prot#:P54646

Uniprot P54646

Gene ID 5563;

Calculated MWPredicted band size: 62 kDa

Sdspage MWObserved band size: 62 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes :AMPK alpha 2(Phospho-S345) Rabbit mAb at 1/500 dilution

Lane 1 : 293T whole cell lysates treated with λpp for 1 hour
Lane 2 : 293T whole cell lysates
Lane 3 : Mouse brain tissue lysates
Lane 4 : Rat brain tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 62 kDa

Exposure time: 12 seconds
Formalin-fixed, paraffin-embedded human pancreas tissue stained for AMPK alpha 2(Phospho-S345) using 13424 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded mouse pancreas tissue stained for AMPK alpha 2(Phospho-S345) using 13424 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence AMPK alpha 2(Phospho-S345) antibody (13424)

ICC/IF staining of AMPK alpha 2(Phospho-S345) in 293 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13424 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence AMPK alpha 2(Phospho-S345) antibody (13424)

ICC/IF staining of AMPK alpha 2(Phospho-S345) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13424 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Five-prime-AMP-activated protein kinase, known as AMPK, is a heterotrimeric complex that comprises of a catalytic α subunit, and regulatory β and γ. AMPK protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. AMPK is activated by high AMP and low ATP via a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase (AMPKK), and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxymethylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 gene maps to chromosome 5p12 and encodes a 548 amino acid protein. The major regulatory site phosphorylated by AMPKK on AMPKα has been identified as Thr 172 within the activation loop between the DFG and APE motifs of the alpha-subunits.

If you have published an article using product 13424, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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