Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > PTEN(Phospho-S380) Rabbit mAb

PTEN(Phospho-S380) Rabbit mAb#13426

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NamePTEN(Phospho-S380) Rabbit mAb

Clone No.JJ08-81

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser380 of human PTEN.

ConjugateUnconjugated

Other Names10q23del antibody
BZS antibody
DEC antibody
GLM2 antibody
MGC11227 antibody
MHAM antibody
MMAC1 antibody
MMAC1 phosphatase and tensin homolog deleted on chromosome 10 antibody
Mutated in multiple advanced cancers 1 antibody
Phosphatase and tensin homolog antibody
Phosphatase and tensin like protein antibody
Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN antibody
Pten antibody
PTEN_HUMAN antibody
PTEN1 antibody
TEP1 antibody

Accession NoSwiss-Prot#:P60484

Uniprot P60484

Gene ID 5728;

Calculated MWPredicted band size: 47 kDa

Sdspage MWObserved band size: 55 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : PTEN(Phospho-S380) Rabbit mAb at 1/1k dilution

Lane 1 : Human lung tissue lysates
Lane 2 : Rat brain tissue lysates
Lane 3 : Mouse brain tissue lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 47 kDa
Observed band size: 55 kDa

Exposure time: 12 seconds
Immunocytochemistry/ Immunofluorescence PTEN(Phospho-S380) antibody (13426)

ICC/IF staining of PTEN(Phospho-S380) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13426 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
The negative control is shown in bottom right hand panel - for the negative control.
Nuclei were counterstained with DAPI.
As human tumors progress to advanced stages, one genetic alteration that occurs at high frequency is a loss of heterozygosity (LOH) at chromosome 10q23. Mapping of homozygous deletions on this chromosome led to the isolation of the PTEN gene, also designated MMAC1 (for mutated in multiple advanced cancers) and TEP1. This candidate tumor suppressor gene exhibits a high frequency of mutations in human glioblastomas and is also mutated in other cancers, including sporadic brain, breast, kidney and prostate cancers. PTEN has been associated with Cowden disease, an autosomal dominant cancer predisposition syndrome. The PTEN gene product is a putative protein tyrosine phosphatase that is localized to the cytoplasm and shares extensive homology with the cytoskeletal proteins tensin and auxilin. Gene transfer studies have indicated that the phosphatase domain of PTEN is essential for growth suppression of glioma cells.

If you have published an article using product 13426, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors