Product Detail
Product NameSHIP (Phospho-Tyr1022) Rabbit mAb
Clone No.JJ082-03
Host SpeciesRabbit
ClonalityMonoclonal
PurificationProA affinity purified
ApplicationsWB, ICC/IF
Species ReactivityHu
Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Tyr1022 of human SHIP.
ConjugateUnconjugated
Other NamesInositol polyphosphate 5 phosphatase of 145kDa antibody
4 antibody
5-trisphosphate 5-phosphatase 1 antibody
hp51CN antibody
Inositol polyphosphate 5 phosphatase 145kDa antibody
Inositol polyphosphate 5 phosphatase antibody
Inositol polyphosphate-5-phosphatase of 145 kDa antibody
INPP5D antibody
MGC104855 antibody
MGC142140 antibody
MGC142142 antibody
p150Ship antibody
Phosphatidylinositol 3,4,5 trisphosphate 5 phosphatase 1 antibody
Phosphatidylinositol-3 antibody
SH2 containing inositol phosphatase isoform b antibody
SH2 domain containing inositol 5' phosphatase 1 antibody
SH2 domain containing inositol phosphatase 1 antibody
SH2 domain-containing inositol phosphatase 1 antibody
SH2 domain-containing inositol-5''-phosphatase 1 antibody
SHIP-1 antibody
SHIP1 antibody
SHIP1_HUMAN antibody
Signaling inositol polyphosphate 5 phosphatase SIP 145 antibody
SIP-145 antibody
SIP145 antibody
Accession NoSwiss-Prot#:Q92835
Uniprot
Q92835
Gene ID
3635;
Calculated MWPredicted band size: 133 kDa
Sdspage MWObserved band size: 145 kDa
Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
StorageStore at -20˚C
Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
All lanes : SHIP(Phospho-Y1020) Rabbit mAb at 1/1k dilution
Lane 1 : Raji whole cell lysates
Lane 2 : Raji treated with pervanadate whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 133 kDa
Observed band size: 145 kDa
Exposure time: 10 seconds
Immunocytochemistry/ Immunofluorescence SHIP(Phospho-Y1020) antibody (13427)
ICC/IF staining of SHIP(Phospho-Y1020) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13427 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The major translational product of the v-Fms oncogene, originally isolated from the McDonough strain of feline sarcoma virus, has been identified as a glycoprotein with intrinsic tyrosine kinase activity. The v-Fms human cellular homolog, c-Fms, has been molecularly cloned and mapped to band q34 on chromosome 5, and identified as the receptor for hematopoietic ligand, CSF-1. Ligand-induced activation of the intrinsic CSF-1R protein tyrosine kinase triggers its interaction with cytoplasmic effector molecules. One such effector molecule, SHIP-1 p145 (SH2-containing-inositol phosphatase), associates with activated Fms. SHIP-1 contains two phosphotyrosine-binding domains (PTB), a unique amino terminal SH2 domain, a proline-rich region, and two highly conserved motifs found among inositol phosphate 5-phosphatases. SHIP-1 displays both phosphatidylinositol 3,4,5-triphosphate and inositol 1,3,4,5-tetrakisphosphate polyphosphate 5-phosphatase activity. Evidence suggests that SHIP-1 may modulate Ras signaling in addition to inositol signaling pathways.
If you have published an article using product 13427, please notify us so that we can cite your literature.
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