Welcome to Sabbiotech!     
Email:info@signalwayantibody.com
Welcome Login Register My Cart(0,$0.00)
Location： Home > Phospho Antibodies > PKC alpha (Phospho-T638) Rabbit mAb

PKC alpha (Phospho-T638) Rabbit mAb#13428

Datasheet PDF
Availability： Yes
Total Amount:$
ADD TO CART
Detail Pictures Description Publish(0) Other
Product Detail

Product NamePKC alpha (Phospho-T638) Rabbit mAb

Clone No.JF0964

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

ConjugateUnconjugated

Other NamesAAG6 antibody
Aging associated gene 6 antibody
aPKC antibody
KPCA_HUMAN antibody
PKC alpha antibody
PKC-A antibody
PKC-alpha antibody
PKCA antibody
PRKACA antibody
PRKCA antibody
Protein Kinase C alpha antibody
Protein kinase C alpha type antibody

Accession NoSwiss-Prot#:P17252

Uniprot P17252

Gene ID 5578;

Calculated MWPredicted band size: 77 kDa

Sdspage MWObserved band size: 80 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : PKC alpha (Phospho-Thr638) Rabbit mAb at 1/1k dilution
Lane 1 : K562 whole cell lysates
Lane 2 : C6 whole cell lysates
Lane 3 : Mouse Brain lysates
Lane 4 : Rat Brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 80 kDa

Exposure time: 3 seconds
All lanes : PKC alpha (Phospho-Thr638) Rabbit mAb at 1/1k dilution
Lane 1 : JK whole cell lysates
Lane 2 : JK treated with 200nM TPA for 30 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 80kDa

Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for PKC alpha (Phospho-T638) using 13428 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence PKC alpha (Phospho-T638) antibody (13428)

ICC/IF staining of PKC alpha (Phospho-T638) in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13428 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence PKC alpha (Phospho-T638) antibody (13428)

ICC/IF staining of PKC alpha (Phospho-T638) in SH-SY-5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13428 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor-promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (α, βI, βII, γ, δ, ε, ζ, η, θ, λ/ι, μ and ν). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC δ and ε are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

If you have published an article using product 13428, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

QUICK NAV

New Products

Cell Signal Transduction

Cancer

Stem Cell

Neuroscience

Help and Support

Contact Us

Terms

Privacy Policy

support

Distributors