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ATM (Phospho-S1981) Rabbit mAb#13432

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Product NameATM (Phospho-S1981) Rabbit mAb

Clone No.JM93-25

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser1981 of human ATM.

ConjugateUnconjugated

Other NamesA-T mutated antibody
A-T mutated homolog antibody
AT mutated antibody
AT1 antibody
ATA antibody
Ataxia telangiectasia mutated antibody
Ataxia telangiectasia mutated gene antibody
Ataxia telangiectasia mutated homolog (human) antibody
Ataxia telangiectasia mutated homolog antibody
ATC antibody
ATD antibody
ATDC antibody
ATE antibody
ATM antibody
ATM serine/threonine kinase antibody
ATM_HUMAN antibody
DKFZp781A0353 antibody
MGC74674 antibody
OTTHUMP00000232981 antibody
Serine protein kinase ATM antibody
Serine-protein kinase ATM antibody
Serine/threonine-protein kinase ATM antibody
Tefu antibody
TEL1 antibody
TEL1, telomere maintenance 1, homolog antibody
TELO1 antibody
Telomere fusion protein antibody

Accession NoSwiss-Prot#:Q13315

Uniprot Q13315

Gene ID 472;

Calculated MWPredicted band size: 351 kDa

Sdspage MWObserved band size: 351 kDa

Formulation1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : ATM (Phospho-S1981) Rabbit mAb at 1/1k dilution

Lane 1 : Hela whole cell lysates
Lane 2 : Hela treated with 1μM Camptothecin for 1 hour whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 351 kDa
Observed band size: 351 kDa

Exposure time: 10 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for ATM (Phospho-S1981) using 13432 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for ATM (Phospho-phospho S1981) using 13432 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence ATM (Phospho- S1981) antibody (13432)

ICC/IF staining of ATM (Phospho- S1981) in HepG2 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13432 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence ATM (Phospho-phospho S1981) antibody (13432)

ICC/IF staining of ATM (Phospho-phospho S1981) in SH-SY5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13432 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Serine / threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thus acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST] -Q. Phosphorylates 'Ser-139' of histone variant H2AX / H2AFX at double strand breaks (DSBs), recover equilibrium DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain Allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin , Soaccessability to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as A genre of activation of ABL1 and SAPK. May play a role in vesicle and / or protein transport. Could play A role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends.

If you have published an article using product 13432, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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