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Location： Home > Phospho Antibodies > alpha Synuclein (Phospho-Ser129) Rabbit mAb

alpha Synuclein (Phospho-Ser129) Rabbit mAb#13434

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Product Namealpha Synuclein (Phospho-Ser129) Rabbit mAb

Clone No.JB22-44

Host SpeciesRabbit

ClonalityMonoclonal

PurificationProA affinity purified

ApplicationsWB, ICC/IF, IHC

Species ReactivityHu, Ms, Rt

Immunogen DescSynthetic phospho-peptide corresponding to residues surrounding Ser129 of human alpha Synuclein.

ConjugateUnconjugated

Other NamesAlpha synuclein antibody
Alpha-synuclein antibody
Alpha-synuclein, isoform NACP140 antibody
alphaSYN antibody
MGC105443 antibody
MGC110988 antibody
MGC127560 antibody
MGC64356 antibody
NACP antibody
Non A beta component of AD amyloid antibody
Non A4 component of amyloid antibody
Non A4 component of amyloid precursor antibody
Non-A beta component of AD amyloid antibody
Non-A-beta component of alzheimers disease amyloid , precursor of antibody
Non-A4 component of amyloid precursor antibody
Non-A4 component of amyloid, precursor of antibody
OTTHUMP00000218549 antibody
OTTHUMP00000218551 antibody
OTTHUMP00000218552 antibody
OTTHUMP00000218553 antibody
OTTHUMP00000218554 antibody
PARK 1 antibody
PARK 4 antibody
PARK1 antibody
PARK4 antibody
Parkinson disease (autosomal dominant, Lewy body) 4 antibody
Parkinson disease familial 1 antibody
SNCA antibody
Snca synuclein, alpha (non A4 component of amyloid precursor) antibody
SYN antibody
Synuclein alpha antibody
Synuclein alpha 140 antibody
Synuclein, alpha (non A4 component of amyloid precursor) antibody
SYUA_HUMAN antibody

Accession NoSwiss-Prot#:P37840

Uniprot P37840

Gene ID 6622;

Calculated MWPredicted band size: 14 kDa

Sdspage MWObserved band size: 18 kDa

FormulationRabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

StorageStore at -20˚C

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : alpha Synuclein (Phospho-Ser129) Rabbit mAb at 1/1k dilution
Lane 1 : Human fetal Brain lysates
Lane 2 : Mouse Brain lysates
Lane 3 : Mouse Brain lysates treated with Lambda Protein Phosphatase for 30min
Lane 4 : Rat Brain lysates
Lane 5 : Rat Brain lysates treated with Lambda Protein Phosphatase for 30min
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 14 kDa
Observed band size: 18 kDa

Exposure time: 9 seconds
Formalin-fixed, paraffin-embedded human brain tissue stained for alpha Synuclein (Phospho-S129) using 13434 at 1/100 dilution in immunohistochemical analysis.The picture on the right is blocked with the phospho peptide.
Immunocytochemistry/ Immunofluorescence alpha Synuclein (Phospho-S129) antibody (13434)

ICC/IF staining of alpha Synuclein (Phospho-S129)in HUVEC cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13434 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
Immunocytochemistry/ Immunofluorescence alpha Synuclein (Phospho-S129) antibody (13434)

ICC/IF staining of alpha Synuclein (Phospho-S129)in SH-SY-5Y cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 13434 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
The synuclein family members, including α-synuclein (also designated NACP for non-β-Amyloid component) and β-synuclein, are predominantly expressed in the brain and are speculated to be involved in synaptic regulation and neuronal plasticity. α-synuclein is localized to neuronal cell bodies and synapses. α-synuclein was first identified as a component of Alzheimer's disease amyloid plaques. Abnormal platelet function in Alzheimer's disease has been demonstrated. During megakaryocytic differentiation α-synuclein has been found to be upregulated, while β-synuclein is downregulated, indicating that coordinate expression of synucleins may be important during hematopoetic cell differentiation. A mutant form of α-synuclein has been found in patients with early onset Parkinson's disease.

If you have published an article using product 13434, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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