Product Detail
Product NameRaf-1 (phospho Ser289) Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsIHC-p,IF(paraffin section),ELISA
Species ReactivityHuman,Mouse,Rat
SpecificityPhospho-Raf-1 (S289) Polyclonal Antibody detects endogenous levels of Raf-1 protein only when phosphorylated at S289.
Immunogen DescThe antiserum was produced against synthesized peptide derived from human C-RAF around the phosphorylation site of Ser289. AA range:251-300
ConjugateUnconjugated
Other NamesRAF1; RAF; RAF proto-oncogene serine/threonine-protein kinase; Proto-oncogene c-RAF; cRaf; Raf-1
Accession NoSwiss Prot:P04049
GeneID:5894
Uniprot
P04049
Gene ID
5894
Calculated MW73kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20˚C/1
Application Details
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/5000. Not yet tested in other applications.
Raf-1 proto-oncogene, serine/threonine kinase(RAF1) Homo sapiens This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq, Jul 2008],
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