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p300 (phospho Ser89) Polyclonal Antibody#13650

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Product Detail

Product Namep300 (phospho Ser89) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsIHC-p,IF(paraffin section),ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-p300 (S89) Polyclonal Antibody detects endogenous levels of p300 protein only when phosphorylated at S89.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human p300 around the phosphorylation site of Ser89. AA range:55-104

ConjugateUnconjugated

Other NamesEP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300

Accession NoSwiss Prot:Q09472
GeneID:2033

Uniprot Q09472

Gene ID 2033

Calculated MW264kd

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details

Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/5000. Not yet tested in other applications.
E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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