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Location： Home > Phospho Antibodies > NOS3 (phospho Ser1177) Polyclonal Antibody

NOS3 (phospho Ser1177) Polyclonal Antibody#13665

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Product Detail

Product NameNOS3 (phospho Ser1177) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IF/ICC,ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-NOS3 (S1177) Polyclonal Antibody detects endogenous levels of NOS3 protein only when phosphorylated at S1177.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human eNOS around the phosphorylation site of Ser1176. AA range:1144-1193

ConjugateUnconjugated

Other NamesNOS3; Nitric oxide synthase; endothelial; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

Accession NoSwiss Prot:P29474
GeneID:4846

Uniprot P29474

Gene ID 4846

Sdspage MW133

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details
Western Blot: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/20000. Not yet tested in other applications.
nitric oxide synthase 3(NOS3) Homo sapiens Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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