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Na+/K+-ATPase α1 (phospho Ser16) Polyclonal Antibody#13687

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Product Detail

Product NameNa+/K+-ATPase α1 (phospho Ser16) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IHC-p,IF(paraffin section),ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-Na+/K+-ATPase α1 (S16) Polyclonal Antibody detects endogenous levels of Na+/K+-ATPase α1 protein only when phosphorylated at S16.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human ATPase around the phosphorylation site of Ser16. AA range:5-54

ConjugateUnconjugated

Other NamesATP1A1; Sodium/potassium-transporting ATPase subunit alpha-1; Na(+)/K(+) ATPase alpha-1 subunit; Sodium pump subunit alpha-1

Accession NoSwiss Prot:P05023
GeneID:476

Uniprot P05023

Gene ID 476

Sdspage MW112

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/5000. Not yet tested in other applications.
ATPase Na+/K+ transporting subunit alpha 1(ATP1A1) Homo sapiens The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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