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Myf-5 (phospho Ser49) Polyclonal Antibody#13694

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Total Amount:$
Product Detail

Product NameMyf-5 (phospho Ser49) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IHC-p,IF(paraffin section),ELISA

Species ReactivityHuman,Mouse

SpecificityPhospho-Myf-5 (S49) Polyclonal Antibody detects endogenous levels of Myf-5 protein only when phosphorylated at S49.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human Myogenic Factor 5 around the phosphorylation site of Ser49. AA range:21-70

ConjugateUnconjugated

Other NamesMYF5; BHLHC2; Myogenic factor 5; Myf-5; Class C basic helix-loop-helix protein 2; bHLHc2

Accession NoSwiss Prot:P13349
GeneID:4617

Uniprot P13349

Gene ID 4617

Sdspage MW38

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/10000. Not yet tested in other applications.
function:Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Efficient DNA binding requires dimerization with another bHLH protein.,

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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