Product Detail
Product NameMDMX (phospho Ser367) Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB,IHC-p,IF(paraffin section),ELISA
Species ReactivityHuman,Mouse,Rat
SpecificityPhospho-MDMX (S367) Polyclonal Antibody detects endogenous levels of MDMX protein only when phosphorylated at S367.
Immunogen DescThe antiserum was produced against synthesized peptide derived from human MDM4 around the phosphorylation site of Ser367. AA range:336-385
ConjugateUnconjugated
Other NamesMDM4; MDMX; Protein Mdm4; Double minute 4 protein; Mdm2-like p53-binding protein; Protein Mdmx; p53-binding protein Mdm4
Accession NoSwiss Prot:O15151
GeneID:4194
Uniprot
O15151
Gene ID
4194
Sdspage MW80
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20˚C/1
Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/5000. Not yet tested in other applications.
MDM4, p53 regulator(MDM4) Homo sapiens This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011],
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