Product Detail
Product NameLIMK-2 (phospho Ser283) Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsWB;IHC;IF;ELISA
Species ReactivityHuman,Mouse,Rat,Monkey
SpecificityPhospho-LIMK-2 (S283) Polyclonal Antibody detects endogenous levels of LIMK-2 protein only when phosphorylated at S283.
Immunogen DescThe antiserum was produced against synthesized peptide derived from human LIMK2 around the phosphorylation site of Ser283. AA range:249-298
ConjugateUnconjugated
Other NamesLIMK2; LIM domain kinase 2; LIMK-2
Accession NoSwiss Prot:P53671
GeneID:3985
Uniprot
P53671
Gene ID
3985
Sdspage MW72
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20˚C/1
Application Details
WB 1:500-1:2000;
IHC 1:100-1:300;
ELISA 1:5000; IF 1:50-200
LIM domain kinase 2(LIMK2) Homo sapiens There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],
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