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LEF-1 (phospho Ser42) Polyclonal Antibody#13745

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Product NameLEF-1 (phospho Ser42) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IF/ICC,ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-LEF-1 (S42) Polyclonal Antibody detects endogenous levels of LEF-1 protein only when phosphorylated at S42.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human LEF-1 around the phosphorylation site of Ser42. AA range:8-57

ConjugateUnconjugated

Other NamesLEF1; Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha

Accession NoSwiss Prot:Q9UJU2
GeneID:51176

Uniprot Q9UJU2

Gene ID 51176

Sdspage MW55

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details
Western Blot: 1/500 - 1/2000.
ELISA: 1/5000. Not yet tested in other applications.
lymphoid enhancer binding factor 1(LEF1) Homo sapiens This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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