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IRF-3 (phospho Ser396) Polyclonal Antibody#13786

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Product NameIRF-3 (phospho Ser396) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-IRF-3 (S396) Polyclonal Antibody detects endogenous levels of IRF-3 protein only when phosphorylated at S396.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human IRF-3 around the phosphorylation site of Ser396. AA range:362-411

ConjugateUnconjugated

Other NamesIRF3; Interferon regulatory factor 3; IRF-3

Accession NoSwiss Prot:Q14653
GeneID:3661

Uniprot Q14653

Gene ID 3661

Sdspage MW60

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details

Western Blot: 1/500 - 1/2000.
ELISA: 1/20000. Not yet tested in other applications.

Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody. The lane on the right is blocked with the phospho peptide.

interferon regulatory factor 3(IRF3) Homo sapiens This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011],

If you have published an article using product 13786, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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