Product Detail
Product NameHistone H3.3 (phospho Ser31) Polyclonal Antibody
Host SpeciesRabbit
ClonalityPolyclonal
PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
ApplicationsIHC-p,IF/ICC,ELISA
Species ReactivityHuman,Mouse,Rat
SpecificityPhospho-Histone H3.3 (S31) Polyclonal Antibody detects endogenous levels of Histone H3.3 protein only when phosphorylated at S31.
Immunogen DescThe antiserum was produced against synthesized peptide derived from human Histone H3.3 around the phosphorylation site of Ser31. AA range:16-65
ConjugateUnconjugated
Other NamesH3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3
Accession NoSwiss Prot:P84243
GeneID:3020/3021
Uniprot
P84243
Gene ID
3020/3021
Calculated MW15kd
Concentration1 mg/ml
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage-20˚C/1
Application Details
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/20000. Not yet tested in other applications.
H3 histone family member 3A(H3F3A) Homo sapiens Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008],
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