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Cytokeratin 18 (phospho Ser33) Polyclonal Antibody#13933

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Product NameCytokeratin 18 (phospho Ser33) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsWB,IHC-p,IF/ICC,ELISA

Species ReactivityHuman,Mouse,Rat

SpecificityPhospho-Cytokeratin 18 (S33) Polyclonal Antibody detects endogenous levels of Cytokeratin 18 protein only when phosphorylated at S33.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human Keratin 18 around the phosphorylation site of Ser33. AA range:1-50

ConjugateUnconjugated

Other NamesKRT18; CYK18; PIG46; Keratin; type I cytoskeletal 18; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

Accession NoSwiss Prot:P05783
GeneID:3875

Uniprot P05783

Gene ID 3875

Sdspage MW47

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details
Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
Immunofluorescence: 1/200 - 1/1000.
ELISA: 1/5000. Not yet tested in other applications.
keratin 18(KRT18) Homo sapiens KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008],

If you have published an article using product 13933, please notify us so that we can cite your literature.

et al,Plakophilin 2 regulates intestinal barrier function by modulating protein kinase C activity in vitro , (2023),
PMID: 36280901

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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