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AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody#14099

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Product Detail

Product NameAMPKα1/2 (phospho Thr183/172) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsIF/ICC,WB,IHC-p,ELISA

Species ReactivityHuman,Mouse,Rat,Monkey,Pig

SpecificityPhospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

ConjugateUnconjugated

Other NamesPRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;

Accession NoSwiss Prot:Q13131/P54646
GeneID:5562/5563

Uniprot Q13131/P54646

Gene ID 5562/5563

Sdspage MW63

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details

IF: 1:50-200 Western Blot: 1/500 - 1/2000.
Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/40000. Not yet tested in other applications.

protein kinase AMP-activated catalytic subunit alpha 1(PRKAA1) Homo sapiens The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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