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AIP4 (phospho Tyr420) Polyclonal Antibody#14108

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Product Detail

Product NameAIP4 (phospho Tyr420) Polyclonal Antibody

Host SpeciesRabbit

ClonalityPolyclonal

PurificationThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

ApplicationsIHC-p,IF(paraffin section),ELISA

Species ReactivityHuman,Mouse

SpecificityPhospho-AIP4 (Y420) Polyclonal Antibody detects endogenous levels of AIP4 protein only when phosphorylated at Y420.

Immunogen DescThe antiserum was produced against synthesized peptide derived from human ITCH around the phosphorylation site of Tyr420. AA range:386-435

ConjugateUnconjugated

Other NamesITCH; E3 ubiquitin-protein ligase Itchy homolog; Itch; Atrophin-1-interacting protein 4; AIP4; NFE2-associated polypeptide 1; NAPP1

Accession NoSwiss Prot:Q96J02
GeneID:83737

Uniprot Q96J02

Gene ID 83737

Calculated MW102kd

Concentration1 mg/ml

FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Storage-20˚C/1

Application Details

Immunohistochemistry: 1/100 - 1/300.
ELISA: 1/20000. Not yet tested in other applications.
itchy E3 ubiquitin protein ligase(ITCH) Homo sapiens This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012],

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NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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