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p53 (Phospho-Ser9) Rabbit mAb#14132

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Product Detail

Product Namep53 (Phospho-Ser9) Rabbit mAb

Clone No.SR1945

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman

SpecificityPhospho-p53 (S9) Antibody detects endogenous levels of total Phospho-p53 (S9)

Immunogen DescA synthesized peptide derived from human Phospho-p53 (S9)

ConjugateUnconjugated

Other NamesCellular tumor antigen p53; Phosphoprotein p53; TP53; Tumor suppressor p53;

Accession NoUniprot:P04637

Uniprot P04637

Calculated MW44 kDa

Sdspage MW53 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000

All lanes : Phospho-p53 (S9) Rabbit mAb at 1/1k dilution

Lane 1 : 293T whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 53 kDa

Exposure time: 15 seconds
All lanes : Phospho-p53 (S9)) Rabbit mAb at 1/1k dilution

Lane 1 : 293T treated with 100nM Calyculin A for 30min whole cell lysates
Lane 2 : 293T whole cell lysates
Lane 3 : 293T treated with Lambda Protein Phosphatase for 30min whole cell lysates

Lysates/proteins at 20 µg per lane.

All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 44 kDa
Observed band size: 53 kDa

Exposure time: 10 seconds

If you have published an article using product 14132, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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