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NF-κB p65 (Phospho-Ser529) Rabbit mAb#14133

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Product Detail

Product NameNF-κB p65 (Phospho-Ser529) Rabbit mAb

Clone No.SR2099

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB,IHC

Species ReactivityHuman

SpecificityPhospho-NF-κB p65 (S529) Antibody detects endogenous levels of total Phospho-NF-κB p65 (S529)

Immunogen DescA synthesized peptide derived from human NF-κB p65

ConjugateUnconjugated

Other NamesNFKB3, Nuclear factor NF-kappa-B p65 subunit, RELA, TF65, Transcription factor p65, p65

Accession NoUniprot:Q04206

Uniprot Q04206

Calculated MWPredicted band size: 65 kDa

Sdspage MWObserved band size: 65 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
IHC: 1:50-1:200
All lanes : NF-κB p65 (Phospho-Ser529) Rabbit mAb at 1/1k dilution
Lane 1 : HeLa whole cell lysates
Lane 2 : HeLa treated with 20ng/ml TNFa for 15min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa

Exposure time: 4 seconds
Formalin-fixed, paraffin-embedded human lung cancer tissue stained for NF-κB p65 (Phospho-Ser529) using 14133 at 1/100 dilution in immunohistochemical analysis.

If you have published an article using product 14133, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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