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Location： Home > Phospho Antibodies > Vimentin (Phospho-Ser72) Rabbit mAb

Vimentin (Phospho-Ser72) Rabbit mAb#14134

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Product Detail

Product NameVimentin (Phospho-Ser72) Rabbit mAb

Clone No.SR1947

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Vimentin (S72) Antibody detects endogenous levels of total Phospho-Vimentin (S72)

Immunogen DescA synthesized peptide derived from human Phospho-Vimentin (S72)

ConjugateUnconjugated

Other NamesVIM; VIME; Vimentin;

Accession NoUniprot:P08670

Uniprot P08670

Calculated MWPredicted band size: 53 kDa

Sdspage MWObserved band size: 53 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : Vimentin (Phospho-Ser72) Rabbit mAb at 1/1k dilution
Lane 1 : HeLa treated with 100nM Calyculin A for 30min whole cell lysates
Lane 2 : 3T3 whole cell lysates
Lysates/proteins at 20 µg per lane.
Lane 3 : 3T3 treated with 100nM Calyculin A for 30min whole cell lysates
Lane 4 : C6 whole cell lysates
Lane 5 : C6 treated with 100nM Calyculin A for 30min whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 6 seconds

If you have published an article using product 14134, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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