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Smad5 (Phospho-Ser463/Ser465) Rabbit mAb#14137

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Product NameSmad5 (Phospho-Ser463/Ser465) Rabbit mAb

Clone No.SR2030

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman Mouse Rat

SpecificityPhospho-Smad5 (S463/465) Antibody detects endogenous levels of total Phospho-Smad5 (S463/465)

Immunogen DescA synthesized peptide derived from human Phospho-Smad5 (S463/465)

ConjugateUnconjugated

Other NameshSmad5; JV5-1 ; MADH5; SMAD5; MusMLP ;SMAD family member 5;

Accession NoUniprot:Q99717

Uniprot Q99717

Calculated MWPredicted band size: 52 kDa

Sdspage MWObserved band size: 58 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes :Smad5 (Phospho-Ser463/Ser465) Rabbit mAb at 1/1k dilution
Lane 1 : HepG2 whole cell lysates
Lane 2 : HepG2 treated with 20 ng/mL TGFβ1 for 15 minutes whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa

Exposure time: 5 seconds
All lanes :Smad5 (Phospho-Ser463/Ser465) Rabbit mAb at 1/1k dilution
Lane 1 : Mouse Brain lysates
Lane 2 : Rat Brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 58 kDa

Exposure time: 4 seconds
Formalin-fixed, paraffin-embedded human pancreas tissue stained for Smad5 (Phospho-Ser463/Ser465) using 14137 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Smad5 (Phospho-Ser463/Ser465) antibody (14137)

ICC/IF staining of (Phospho-Ser463/Ser465) in 293T cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14137 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14137, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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