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Phospho Antibodies > Retinoblastoma (Phospho-Ser780) Rabbit mAb
Retinoblastoma (Phospho-Ser780) Rabbit mAb#14147
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All lanes : Retinoblastoma (Phospho-Ser780) Rabbit mAb at 1/1k dilutionLane 1 :Jurkat treated with Lambda phosphatase for 30min cell lysate Lane 2 :Jurkat cell lysatelysate/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 106 kDa Observed band size: 110 kDaExposure time: 7 seconds
All lanes : Retinoblastoma (Phospho-Ser780) Rabbit mAb at 1/1k dilutionLane 1 :THP-1 cell lysate Lane 2 :SH-SY5Y cell lysateLane 3 :A673 cell lysateLane 4 :Hela cell lysatelysate/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 106 kDa Observed band size: 110 kDaExposure time: 10 seconds
Formalin-fixed, paraffin-embedded human tonsil tissue stained for Retinoblastoma (Phospho-Ser780) using 14147 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence Retinoblastoma (Phospho-Ser780) antibody (14147)
ICC/IF staining of Retinoblastoma (Phospho-Ser780) in Hela cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14147 (red) at a working dilution of 1/100.GFAP Monoclonal antibody (green) was diluted at 1:200.
Goat Anti Rabbit Alexa Fluor 647 was diluted at 1:1000. Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
