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Location： Home > Phospho Antibodies > S6K1 (Phospho-Thr421/Ser424) Rabbit mAb

S6K1 (Phospho-Thr421/Ser424) Rabbit mAb#14148

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Product NameS6K1 (Phospho-Thr421/Ser424) Rabbit mAb

Clone No.5A3

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB

Species ReactivityHuman Rat

SpecificityPhospho-S6K1 (T421 + S424) Antibody detects endogenous levels of total Phospho-S6K1 (T421 + S424)

Immunogen DescA synthesized peptide derived from human Phospho-S6K1 (T421 + S424)

Target NameS6K1

ConjugateUnconjugated

Other NamesEC 2.7.11.1, KS6B1, P70-S6K, RPS6KB1, Ribosomal protein S6 kinase, Ribosomal protein S6 kinase, 70kDa, polypeptide 1, S6K, kinase p70S6K, p70-S6K

Accession NoUniprot:P23443

Uniprot P23443

Calculated MWPredicted band size: 59 kDa

Sdspage MWObserved band size: 70 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
All lanes : S6K1 (Phospho-Thr421/Ser424) Rabbit mAb at 1/1k dilution
Lane 1 :Western blot analysis of SK61 phosphorylation expression in HEK293 cell lysate
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 59 kDa
Observed band size: 70 kDa
Exposure time: 9 seconds

If you have published an article using product 14148, please notify us so that we can cite your literature.

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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