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Location： Home > Phospho Antibodies > STAT3 (Phospho-Ser727) Rabbit mAb

STAT3 (Phospho-Ser727) Rabbit mAb#14152

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Product Detail

Product NameSTAT3 (Phospho-Ser727) Rabbit mAb

Clone No.SR2312

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB, ICC/IF, IHC

Species ReactivityHuman Mouse Rat

SpecificityPhospho-STAT3 (S727) Antibody detects endogenous levels of total Phospho-STAT3 (S727)

Immunogen DescA synthesized peptide derived from human Phospho-STAT3 (S727)

ConjugateUnconjugated

Other NamesAPRF;Acute-phase response factor;Signal transducer and activator of transcription 3;Stat3;

Accession NoUniprot:P40763

Uniprot P40763

Calculated MWPredicted band size: 88 kDa

Sdspage MWObserved band size: 98 kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details
WB: 1:500-1:2000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
All lanes : STAT3 (Phospho-Ser727) Rabbit mAb at 1/1k dilution
Lane 1 :A431 cell lysate
Lane 2 :A431 cell lysate treated with 100ng/ml EGF 10min
lysate/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilution
Predicted band size: 88 kDa
Observed band size: 98 kDa
Exposure time: 5 seconds
Formalin-fixed, paraffin-embedded rat brain tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded rat kidney tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human brain tissue stained for STAT3 (Phospho-Ser727) using 14152 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence STAT3 (Phospho-Ser727) antibody (14152)

ICC/IF staining of STAT3 (Phospho-Ser727) in A431 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14152 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.

If you have published an article using product 14152, please notify us so that we can cite your literature.

et al,Effects of Rituximab on JAK-STAT and NF-κB signaling pathways in acute lymphoblastic leukemia and chronic lymphocytic leukemia , (2019),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
;

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