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KD/KO Validation > Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb
Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb#14158
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All lanes : Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb at 1/1k dilutionLane 1 : 3T3 whole cell lysatesLane 2 : 3T3 treated with Lambda Protein Phosphatase for 30min whole cellLane 3 : Hela whole cell lysatesLane 4 : Hela treated with Lambda Protein Phosphatase for 30min whole cellLysates/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 84 kDa Observed band size: 85 kDaExposure time: 5 seconds
All lanes : Glycogen synthase 1 (Phospho-Ser641) Rabbit mAb at 1/1k dilution
Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : Glycogen synthase 1 (Phospho-Ser641) knockdown HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Formalin-fixed, paraffin-embedded human colon tissue stained for Glycogen synthase 1 (Phospho-Ser641) using 14158 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence Glycogen synthase 1 (Phospho-Ser641) antibody (14158) ICC/IF staining of Glycogen synthase 1 (Phospho-Ser641) in A549 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100. Samples were incubated with 14158 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 647 goat anti rabbit, used at a dilution of 1/500. Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
