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FAK (Phospho-Tyr397) Rabbit mAb#14175

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Product Detail

Product NameFAK (Phospho-Tyr397) Rabbit mAb

Host SpeciesRabbit

ClonalityMonoclonal

IsotypeRabbit IgG

PurificationAffinity-chromatography

ApplicationsWB ICC/IF

Species ReactivityHuman Mouse Rat

SpecificityPhospho-FAK (Y397) Antibody detects endogenous levels of Phospho-FAK (Y397)

Immunogen DescA synthesized peptide derived from human Phospho-FAK (Y397)

ConjugateUnconjugated

Other NamesEC 2.7.10.2; FADK 1; FAK1; Focal adhesion kinase 1; PTK2; Protein- tyrosine kinase 2; pp125FAK;

Accession NoUniprot:Q05397

Uniprot Q05397

Calculated MW119kDa

FormulationRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageStore at +4˚C short term. Store at -20˚C long term. Avoid freeze / thaw cycle.

Application Details

WB:1:500~1:2000
ICC/IF:1:50~1:200

Western blot analysis of Phospho-FAK (Y397) expression in EGF treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.

If you have published an article using product 14175, please notify us so that we can cite your literature.

et al,Spatial dimension cues derived from fibrous scaffolds trigger mechanical activation to potentiate the paracrine and regenerative functions of MSCs via the FAK-PI3K/AKT axis , (2024),
PMID:

NOTE

Application

  • WBWestern Blotting
  • IHCImmunohistochemistry
  • IFImmunofluorescence
  • ICCImmunocytochemistry
  • FCFlow Cytometry
  • IPImmunoprecipitation
  • EELISA
  • DBDot Blotting
  • ChIPChromatin Immunoprecipitation
  • GICAGold Immunochromatography Assay
  • NCNegative Control

Species Reactivity

  • HuHuman
  • MsMouse
  • RtRat
  • DmDrosophila melanogaster
  • CCaenorhabditis elegans
  • MkMonkey
  • RbRabbit
  • BBovine
  • DDog
  • PPig
  • HmHamster
  • ChHmChinese Hamster
  • ChkChicken
  • ShpSheep
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