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Phospho Antibodies > ER alpha (Phospho-Ser118) Rabbit mAb
ER alpha (Phospho-Ser118) Rabbit mAb#14181
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All lanes : ER alpha (Phospho-Ser118) Rabbit mAb at 1/1k dilutionLane 1 :MCF7 cell lysateLane 2 : MCF7 cell lysate treated with b-Estradiol and EGF. lysate/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 66 kDa Observed band size: 66 kDaExposure time: 9 seconds
All lanes : ER alpha (Phospho-Ser118) Rabbit mAb at 1/1k dilutionLane 1 :Hela cell lysateLane 2 : Mouse brain lysate Lane 3 : Rat brain lysatelysate/proteins at 20 µg per lane.SecondaryAll lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/20000 dilutionPredicted band size: 66 kDa Observed band size: 66 kDaExposure time: 12 seconds
Formalin-fixed, paraffin-embedded human kidney tissue stained for ER alpha (Phospho-Ser118) using 14181 at 1/100 dilution in immunohistochemical analysis.
Immunocytochemistry/Immunofluorescence Retinoblastoma ER alpha (Phospho-Ser118) antibody (14181)
ICC/IF staining of ER alpha (Phospho-Ser118) in MCF7 cells. Cells were fixed with 4% Paraformaldehyde permeabilized with 0.1% Triton X-100.
Samples were incubated with 14181 at a working dilution of 1/100. The secondary antibody was Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1/500.
Nuclei were counterstained with DAPI.
NOTE
Application
- WBWestern Blotting
- IHCImmunohistochemistry
- IFImmunofluorescence
- ICCImmunocytochemistry
- FCFlow Cytometry
- IPImmunoprecipitation
- EELISA
- DBDot Blotting
- ChIPChromatin Immunoprecipitation
- GICAGold Immunochromatography Assay
- NCNegative Control
Species Reactivity
- HuHuman
- MsMouse
- RtRat
- DmDrosophila melanogaster
- CCaenorhabditis elegans
- MkMonkey
- RbRabbit
- BBovine
- DDog
- PPig
- HmHamster
- ChHmChinese Hamster
- ChkChicken
- ShpSheep
